Dionex CarboPac PA20 - High-Resolution Column for Carbohydrate and Sugar Analysis

The Dionex CarboPac PA20 is a strong anion-exchange column specifically designed for high-resolution separation of mono- and disaccharides using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD). No sample derivatization is required.

Key Specifications:

• Manufacturer: Thermo Scientific (Dionex)
• Column Type: Strong anion-exchange, pellicular
• Substrate: 6.5 µm diameter nonporous beads with MicroBead latex
• Dimensions: 3 x 150 mm (analytical), 0.4 x 150 mm (capillary), 3 x 30 mm (fast sialic acid)
• Max Pressure: 3500 psi
• Solvent Compatibility: pH 0–14, 100% HPLC solvents
• Part Number: 060142

Why Choose the CarboPac PA20?

The CarboPac PA20 achieves baseline resolution between the six monosaccharides commonly found in mammalian glycoproteins — fucose, galactosamine, glucosamine, galactose, glucose, and mannose — without any sample derivatization. This direct detection approach saves sample preparation time and eliminates derivatization artifacts.

The 6.5 µm pellicular resin structure provides excellent mass transfer, resulting in high chromatographic efficiency and rapid column re-equilibration between injections.

Applications:

• Glycoprotein monosaccharide composition analysis
• Biopharmaceutical glycan characterization
• Food and beverage sugar profiling (juice, dairy, honey)
• Dietary fiber carbohydrate composition
• Oligosaccharide mapping
• Sialic acid determination (using 3 x 30 mm fast format)

CarboPac PA20 vs. PA1 vs. PA10:

The PA20 provides slightly different selectivity from the PA1 and PA10, making it especially useful for resolving difficult peak pairs. The PA20 is the recommended first choice for high-sensitivity monosaccharide analysis in glycoprotein therapeutics.

Recommended System Configuration:

• Guard Column: CarboPac PA20 Guard (3 x 30 mm, P/N 060144)
• Detection: Pulsed Amperometric Detection (PAD) with gold electrode
• Eluent Generator: EGC 500 KOH
• System: Dionex ICS-5000+, ICS-6000

Order from Eke Lab Shop. Contact us for application support and system configuration.